{"id":3219,"date":"2012-11-17T15:39:52","date_gmt":"2012-11-17T22:39:52","guid":{"rendered":"http:\/\/elbertcounty.net\/blog\/2012\/11\/17\/gnome-laser-transfection\/"},"modified":"2012-11-17T15:42:52","modified_gmt":"2012-11-17T22:42:52","slug":"gnome-laser-transfection","status":"publish","type":"post","link":"https:\/\/elbertcounty.net\/blog\/2012\/11\/gnome-laser-transfection\/","title":{"rendered":"Gnome Laser Transfection"},"content":{"rendered":"<p>&#8220;Areas of application<\/p>\n<p>An efficient and gentle cell transfection of sensitive cells is crucial for gene therapy and other biomedical applications.\u00a0 Particularly alternative non-viral delivery-systems are needed to avoid the risks associated with viral transduction.\u00a0 To address these requirements we have developed a technique termed gold nanoparticle mediated [GNOME] laser transfection: Gold nanoparticles are in close vicinity to the cell membrane and irradiated by a weakly focused laser beam.\u00a0 The particle-laser interaction leads to plasmonic effects which in turn can evoke transient cell membrane permeabilization, allowing diffusion of extracellular molecules into the cytoplasm.<\/p>\n<p>GNOME laser transfection thus provides delivery of a broad range of molecules into different cell types, e.g. primary neurons, stem cells and iPS cells.\u00a0 Concurrently it has a low toxicity and offers high sample throughput.&#8221;<\/p>\n<p>www.lzh.de<br \/>\nwww.lasermedizin.uni-hannover.de<br \/>\nwww.rebirth-hanover.de<\/p>\n<p>Translation, if you have a genetic deficiency, perhaps are genetically disposed to a certain cancer, or maybe you have nerve damage that left you with a physical incapacity, it sounds like they can now fix these things.<\/p>\n<p>B_Imperial<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#8220;Areas of application An efficient and gentle cell transfection of sensitive cells is crucial for gene therapy and other biomedical applications.\u00a0 Particularly alternative non-viral delivery-systems are needed to avoid the risks associated with viral transduction.\u00a0 To address these requirements we have developed a technique termed gold nanoparticle mediated [GNOME] laser transfection: Gold nanoparticles are in [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"nf_dc_page":"","_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[113],"tags":[],"class_list":{"0":"post-3219","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-tech","7":"entry"},"_links":{"self":[{"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/posts\/3219","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/comments?post=3219"}],"version-history":[{"count":0,"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/posts\/3219\/revisions"}],"wp:attachment":[{"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/media?parent=3219"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/categories?post=3219"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/elbertcounty.net\/blog\/wp-json\/wp\/v2\/tags?post=3219"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}